Increased Recombination Intermediates and Homologous Integration Hot Spots at DNA Replication Origins.
Segurado M, Gomez M, Antequera F
Molecular Cell (2002)
Category: DNA replication ¤ Added: Oct 30, 2002 ¤ Rating: ◊◊
We have studied the relationship between DNA replication and recombination in Schizosaccharomyces pombe using two-dimensional gel electrophoresis and functional analysis. Our results indicate that the activation of replication origins (ORIs) during the mitotic cell cycle is associated with the generation of joint DNA molecules between sister chromatids. The frequency of integration by homologous recombination was up to 50-fold higher than the genomic average within a narrow window overlapping the ars1 replication initiation site. The S. pombe rad22-, rhp51-, and rhp54- mutants, deficient in mitotic recombination, activate ORIs very inefficiently and accumulate abnomal replication intermediates. These results focus on the general link between replication and recombination previously found in several systems and suggest a role for recombination in the initiation of eukaryotic DNA replication.