|Targeted Stimulation of Meiotic Recombination.|
Pecina A, Smith KN, Mezard C, Murakami H, Ohta K, Nicolas A
Category: meiosis ¤ Added: Oct 22, 2002 ¤ Rating: ◊◊
Meiotic recombination in Saccharomyces cerevisiae is initiated by programmed DNA double-strand breaks (DSBs), a process that requires the Spo11 protein. DSBs usually occur in intergenic regions that display open chromatin accessibility, but other determinants that control their frequencies and non-random chromosomal distribution remain obscure.We report that a Spo11 construct bearing the Gal4 DNA binding domain not only rescues spo11- spore inviability and catalyzes DSB formation at natural sites but also strongly stimulates DSB formation near Gal4 binding sites. At GAL2, a naturally DSB-cold locus, Gal4BD-Spo11 creates a recombinational hotspot that depends on all the other DSB gene functions, showing that the targeting of Spo11 to a specific site is sufficient to stimulate meiotic recombination that is under normal physiological control.