Yeast Mon1p/Aut12p functions in vacuolar fusion of autophagosomes and cvt-vesicles.
Meiling-Wesse K, Barth H, Voss C, Barmark G, Murén E, Ronne H, Thumm M
FEBS Letters (2002)
Category: autophagy, vacuole ¤ Added: Feb 06, 2009 ¤ Rating: ◊◊
Here we identify Mon1p as being essential for the cvt-pathway and autophagy. Thus, mon1Delta cells are impaired in proaminopeptidase I maturation and homozygous diploid mon1Delta cells do not sporulate. Quantitative autophagy measurements suggest a complete autophagy block. The autophagosomal marker protein GFP-Aut7p accumulates in mon1Delta cells at punctate structures outside the vacuole. Furthermore, proaminopeptidase I accumulates in mon1Delta cells in a proteinase-protected form. Our data demonstrate that mon1Delta cells are defective in the fusion of cvt-vesicles and autophagosomes with the vacuole. Consistent with this, GFP-Mon1p localizes to the cytosol and to punctate structures within the cytosol.