Non-random segregation of sister chromosomes in Escherichia coli.
White M, Eykelenboom JK, Lopez-Vernaza MA, Wilson E, Leach DRF
Nature (2008)
Category: bacterial chromosome, DNA replication ¤ Added: Oct 30, 2008 ¤ Rating: ◊◊
It has long been known that the 59 to 39 polarity of DNA synthesis results in both a leading and lagging strand at all replication forks1. Until now, however, there has been no evidence that leading or lagging strands are spatially organized in any way within a cell. Here we show that chromosome segregation in Escherichia coli is not random but is driven in a manner that results in the leading and lagging strands being addressed to particular cellular destinations. These destinations are consistent with the known patterns of chromosome segregation2,3. Our work demonstrates a new level of organization relating to the replication and segregation of the E. coli chromosome.