Novel method for evaluation of the oligomeric structure of membrane proteins.
Ramjeesingh M, Huan LJ, Garami E, Bear CE
Biochemical Journal (1999)
Category: methods ¤ Added: Apr 18, 2008 ¤ Rating: ◊◊
Assessment of the quaternary structure of membrane proteins by PAGE has been problematic owing to their relatively poor solubility in non-dissociative detergents. Here we report that several membrane proteins can be readily solubilized in their native quaternary structure with the use of the detergent perfluoro-octanoic acid (PFO). Further, PFO can be used with PAGE, thereby providing a novel, accessible tool with which to assess the molecular mass of homo-multimeric protein complexes.
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