Identification and characterization of an essential telomeric repeat binding factor in Schizosaccharomyces pombe [Manuscript]
Pitt CW, Valente LP, Rhodes D, Simonsson T
Journal of Biological Chemistry (2007)
Category: telomere ¤ Added: Nov 01, 2007 ¤ Rating: ◊◊
While mammalian cells harbor two double strand telomeric repeat binding factors, TRF1 and TRF2, the fission yeast Schizosaccharomyces pombe has been thought to harbor solely the TRF1/TRF2 ortholog Taz1p to perform comparable functions. Here we report the identification of telomeric repeat binding factor 1 (Tbf1), a second TRF1/TRF2 ortholog in Schizosaccharomyces pombe. Like the Taz1p, the identified Tbf1p shares amino acid sequence similarity, as well as structural and functional characteristics, with the mammalian TRF1 and TRF2 proteins. This family of proteins shares a common architecture with two separate structural domains. An N-terminal domain is necessary and sufficient for the formation of homodimers, and a C-terminal MYB/homeodomain mediates sequence specific recognition of double-stranded telomeric DNA. The identified Tbf1p binds Schizosaccharomyces pombe telomeric DNA with high sequence specificity in vitro. Targeted deletion of the tbf1 gene reveals that it is essential for survival, and over-expression of the tbf1 gene leads to telomere elongation in vivo, which is dependent upon the MYB domain. These data suggests that fission yeast, like mammals, have two factors that bind double stranded telomeric DNA and perform distinct roles in telomere length regulation.