The Saccharomyces cerevisiae Fin1 protein forms cell cycle-specific filaments between spindle pole bodies.
van Hemert MJ, Lamers GE, Klein DC, Oosterkamp TH, Steensma HY, van Heusden GP
Proceedings of National Academy of Sciences USA (2002)
Category: cytoskeleton ¤ Added: Jun 13, 2002 ¤ Rating: ◊◊
The FIN1 gene from the yeast Saccharomyces cerevisiae encodes a basic protein with putative coiled-coil regions. Here we show that in large-budded cells a green fluorescent protein-Fin1 fusion protein is visible as a filament between the two spindle pole bodies. In resting cells the protein is undetectable, and in small-budded cells it is localized in the nucleus. During late mitosis it localizes on the spindle pole bodies. Filaments of cyano fluorescent protein-tagged Fin1 colocalize with filaments of green fluorescent protein-tagged Tub1 only in large-budded cells. By electron and atomic force microscopy we showed that purified recombinant Fin1p self-assembles into filaments with a diameter of approximately 10 nm. Our results indicate that the Fin1 protein forms a cell cycle-specific filament, additional to the microtubules, between the spindle pole bodies of dividing yeast cells.