Tiny telomere DNA.
Ren J, Qu X, Trent JO, Chaires JB
Nucleic Acids Research (2002)
Category: telomere ¤ Added: Jun 07, 2002 ¤ Rating: ◊◊
We describe the design, synthesis and biophysical characterization of a novel DNA construct in which a folded quadruplex structure is joined to a standard double helix. Circular dichroism, gel electrophoresis, three-dimensional UV melting and differential scanning calorimetry were all used to characterize the structure. Rigorous molecular dynamics simulations were used to build a plausible atomic-level structural model of the DNA construct. This novel DNA construct provides a model for the duplex-quadruplex junction region at the end of chromosomal DNA and offers a system for the study of structure-selective ligand binding