|Different role of functional domains of hTR in DNA binding to telomere and telomerase reconstruction.|
Yeo M, Rha SY, Jeung HC, Shen XH, Yang SH, An SW, Roh JK, Chung HC
FEBS Letters (2005)
Category: telomerase, telomere ¤ Added: Jan 03, 2005 ¤ Rating: ◊◊
Even if template sequence of hTR played an essential role in telomere binding, a 326 nucleotide fragment of hTR containing template, pseudoknot, and CR4-5 domains is critical for both binding with telomeric DNA and reconstitution of telomerase activity. A functional study with antisense oligonucleotides suggested that targeted disruption of the template region efficiently abrogated both telomeric DNA binding and telomerase activity, whereas disruption of the CR4-5 region induced only loss of telomerase activity. hTR interacts with telomeric DNA via structural region composed of the template, pseudoknot, and CR4-5 domains, however, each structural domain plays a distinct role in telomere binding and telomerase activity reconstitution.