Characterization of Single Stranded Telomeric DNA-binding Proteins in Cultured Soybean (Glycine max) Cells.
Kwon C, Kwon K, Chung IK, Kim SY, Cho MH, Kang BG
Molecules and Cells (2004)
Category: telomere ¤ Added: Jul 08, 2004 ¤ Rating: ◊◊
We have identified and characterized a protein factor in soybean (Glycine max) nuclear extracts that binds to plant single stranded telomeric DNA repeats. A single DNA-protein complex was detected in gel retardation assays using synthetic telomeres and nuclear extracts. The protein forming this complex was designated soy-bean (Glycine max) single stranded telomeric DNA-binding protein (Gm-STBP). Gm-STBP binds to single stranded telomeric DNA containing more than two repeats. It does not bind to Tetrahymena, human or mutated plant telomere sequences, and its binding ac-tivity is not affected by RNase treatment. Gm-STBP activity gradually decreased after suspension cultures entered stationary phase. A slower migrating band was formed with extracts of earlier and later phases of soybean suspension cultures. Our findings suggest that binding of Gm-STBP to plant single stranded telom-eric DNA may play a role in the proper functioning of telomeres during development.