|Intramitochondrial Localization of Universal Minicircle Sequence-binding Protein, a Trypanosomatid Protein that Binds Kinetoplast Minicircle Replication Origins.|
Abu-Elneel K, Robinson DR, Drew ME, Englund PT,Shlomai J
Journal of Cell Biology (2001)
Category: mitochondrial DNA ¤ Added: Nov 13, 2002 ¤ Rating: ◊◊
Abstract. Kinetoplast DNA (kDNA), the mitochondrial DNA of the trypanosomatid Crithidia fasciculata , is a unique structure containing 5,000 DNA minicircles topologically linked into a massive network. In vivo, the network is condensed into a disk-shaped structure. Replication of minicircles initiates at unique origins that are bound by universal minicircle sequence (UMS)- binding protein (UMSBP), a sequence-specific DNAbinding protein. This protein, encoded by a nuclear gene, localizes within the cells single mitochondrion. Using immunofluorescence, we found that UMSBP localizes exclusively to two neighboring sites adjacent to the face of the kDNA disk nearest the cells flagellum. This site is distinct from the two antipodal positions at the perimeter of the disk that is occupied by DNA polymerase b , topoisomerase II, and a structure-specific endonuclease. Although we found constant steady-state levels of UMSBP mRNA and protein and a constant rate of UMSBP synthesis throughout the cell cycle, immunofluorescence indicated that UMSBP localization within the kinetoplast is not static. The intramitochondrial localization of UMSBP and other kDNA replication enzymes significantly clarifies our understanding of the process of kDNA replication.